TOOLBOX
Lab work / experimental setup
Introducing people from different backgrounds to the experimental process, laboratory practices and exploratory techniques might be a challenging but powerful education tool. These knowledges and techniques will allow non-scientists to enlarge their field of action giving them new avenues to explore and create. Meanwhile scientists will be confronted with the necessity to communicate their field and rethink their practices.
DNA and RNA Degradation
It is a Lab procedure
to show what is DNA/RNA degradation.
FOR WHOM:
This tool is targeted to Higher Education students but must be used with the supervision of a teacher and/or technician with experience in molecular biology laboratory techniques.
WHAT FOR:
This tool is useful
in lab settings as an example of good practices to work with DNA / RNA, two molecules with high sensitivity for degradation.
WHERE TO APPLY IT: The tool can be applied to illustrate to Higher Education students the relevancy of laboratory good practices while working with RNA and DNA.
Single guide RNA synthesis
This is a Lab protocol used to do the synthesis of sgRNA with EnGen sgRNA Synthesis Kit.
FOR WHOM:
This tool is targeted to Higher Education students but must be used with the supervision of a teacher and/or technician with experience in molecular biology laboratory techniques.
WHAT FOR:
This tool is useful in genetic molecular lab settings to do the synthesis of a sgRNA with EnGen sgRNA Synthesis kit (in vitro) and confirm the RNA synthesis through running sgnRA in a gel.
WHERE TO APPLY IT: The tool can be used in an exercise to learn and understand the process of CRISPR–Cas9 genome-editing technology to design target-specific oligo.
In vitro cleavage of target DNA
This is a Lab protocol used in CRISPR–Cas9 genome-editing technology.
FOR WHOM:
This tool is targeted to Higher Education students but must be used with the supervision of a teacher and/or technician with experience in molecular biology laboratory techniques.
WHAT FOR:
This tool is useful to guide students /teachers in the process of in vitro cleavage of target DNA, while editing a genome with CRISPR-Cas9.
WHERE TO APPLY IT: The tool can be applied as a practical lab exercise for STEAM students. It's a Lab protocol used in CRISPR–Cas9 genome-editing technology.
Gel Electrophoresis
This is a lab protocol that describes the method used to measure and sort strands of DNA.
FOR WHOM:
This tool is targeted to Higher Education students but must be used with the supervision of a teacher and/or technician with experience in molecular biology laboratory techniques.
WHAT FOR:
This tool is useful in a laboratory setting to measure and sort strands of DNA or separate individual proteins.
WHERE TO APPLY IT: This tool can be used to introduce STEAM students to laboratory techniques used in a molecular biology lab, including in CRISPR–Cas9 genome-editing.
RNA purification with Spin column
This is a Lab protocol used in CRISPR–Cas9 genome-editing technology to purify RNA.
FOR WHOM:
This tool is targeted to Higher Education students but must be used with the supervision of a teacher and/or technician with experience in molecular biology laboratory techniques.
WHAT FOR:
This tool is useful in a lab setting to purify sgRNA using a spin column and run the purified sgRNA to confirm recovery of RNA and quantification.
WHERE TO APPLY IT: The tool can be applied as a practical lab exercise for STEAM students. It's a Lab protocol used in CRISPR–Cas9 genome-editing technology.
Creative Tools to Generate AI Art
FOR WHOM:
This resource is indicated to Higher Education students with some knowledge of computer programming, or in teams with mixed backgrounds.
WHAT FOR:
This resource is useful to explore the potential of AI in artistic practices and creative set-ups.
WHERE TO APPLY IT: This website lists and describes a series of existing AI tools that can be used in artistic and creative settings. The students can explore some of the tools to create their own projects.